Effect of polyphyllin D on proliferation and apoptosis of human pancreatic cancer cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1418-1422, 2020.
Article
em Zh
| WPRIM
| ID: wpr-1008587
Biblioteca responsável:
WPRO
ABSTRACT
Polyphyllin D is a steroid saponin monomer in Polyphyllin, with antibacterial, analgesic, sedative, anti-tumor and other pharmacological effects, but is rarely reported in pancreatic cancer. This study detected apoptosis-relevant indicators, in order to explore the effect of polyphyllin D on the proliferation and apoptosis of human pancreatic cancer Panc-1 cells and relevant mechanisms of action. After pancreatic cancer Panc-1 cells were treated with polyphyllin D(0, 1, 2, 3, 4, 5 μg·μL~(-1)) for 24, 48 and 72 hours, CCK-8 method was used to detect the effect of polyphyllin D on the proliferation of pancreatic cancer Panc-1 cells. Flow cytometry was used to detect cell cycle and changes in mitochondrial membrane potential(MMP). The apoptosis was detected by Annexin V-FITC/PI staining, and Western blot was used to detect the protein expressions of cytochrome C(Cyto C), Bax, Bcl-2, cleaved caspase-3 and cleaved caspase-9. The results indicated that compared with the control group, polyphyllin D could inhibit the proliferative activity of Panc-1 cells in a time and concentration-dependent manner. Flow cytometry results showed that polyphyllin D could block the cells in S and G_2/M phase in a concentration manner, the MMP of the cells was significantly reduced, and the apoptosis rate increased with the concentration of polyphyllin D. Western blot results showed that polyphyllin D could concentration-dependently up-regulate the protein expression levels of Bax, Cyto C, cleaved caspase-3 and cleaved caspase-9, and down-regulate the protein expression level of Bcl-2. The above findings suggested that polyphyllin D could effectively inhibit the proliferation of Panc-1 cells, and its mechanism may be related to the blocking of cell growth cycle and the apoptosis induced by mitochondrial pathway.
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Índice:
WPRIM
Assunto principal:
Neoplasias Pancreáticas
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Saponinas
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Apoptose
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Proteínas Proto-Oncogênicas c-bcl-2
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Linhagem Celular Tumoral
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Proliferação de Células
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Diosgenina
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Proteína X Associada a bcl-2
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Caspase 3
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Caspase 9
Limite:
Humans
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2020
Tipo de documento:
Article