Comparison of two luminescence detection methods for staphylococcal enterotoxin C content in simulated milk samples / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
; (12): 1089-1093, 2023.
Article
em Zh
| WPRIM
| ID: wpr-1009459
Biblioteca responsável:
WPRO
ABSTRACT
Objective To compare the sensitivity and accuracy of amplified luminescent proximity homogeneous assay linked immunosorbent assay (AlphaLISA) and magnetic particles-based chemiluminescence immunoassay (MP-CLIA) for detection of staphylococcal enterotoxin C (SEC) in the simulated milk samples. Methods The AlphaLISA was constructed using goat anti-SEC polyclonal antibody-coupled receptor microspheres, biotin-labeled SEC monoclonal antibody and streptavidin-coupled donor microspheres. The MP-CLIA was constructed using goat anti-SEC polyclonal antibody conjugated alkaline phosphatase, biotin-labeled anti-SEC monoclonal antibody and streptavidin conjugated magnetic beads. Results The sensitivity of AlphaLISA to detect SEC content in simulated milk samples was 4.04 ng/L, and the coefficient of variation (CV) was 1.98%~9.82%. The sensitivity of MP-CLIA was 108.19 ng/L and CV was 4.63%~20.40%. Conclusion Compared with MP-CLIA, AlphaLISA is more sensitive and accurate to detecting SEC.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Biotina
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Cabras
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Imunoensaio
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Estreptavidina
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Leite
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Luminescência
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Anticorpos Monoclonais
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Cellular and Molecular Immunology
Ano de publicação:
2023
Tipo de documento:
Article