Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 315-326, 2020.
Article
em En
| WPRIM
| ID: wpr-1010537
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#Drug-resistance and metastasis are major reasons for the high mortality of ovarian cancer (OC) patients. Cyclooxygenase-2 (COX-2) plays a critical role in OC development. This study was designed to evaluate the effects of COX-2 on migration and cisplatin (cis-dichloro diammine platinum, CDDP) resistance of OC cells and explore its related mechanisms.@*METHODS@#Cell counting kit-8 (CCK-8) assay was used to detect the cytotoxicity effects of celecoxib (CXB) and CDDP on SKOV3 and ES2 cells. The effect of COX-2 on migration was evaluated via the healing test. Western blot and real-time quantitative polymerase chain reaction (qPCR) were used to analyze E-cadherin, vimentin, Snail, and Slug levels.@*RESULTS@#COX-2 promoted drug-resistance and cell migration. CXB inhibited these effects. The combination of CDDP and CXB increased tumor cell sensitivity, reduced the amount of CDDP required, and shortened treatment administration time. COX-2 upregulation increased the expression of Snail and Slug, resulting in E-cadherin expression downregulation and vimentin upregulation.@*CONCLUSIONS@#COX-2 promotes cancer cell migration and CDDP resistance and may serve as a potential target for curing OC.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Neoplasias Ovarianas
/
Movimento Celular
/
Reação em Cadeia da Polimerase
/
Cisplatino
/
Resistencia a Medicamentos Antineoplásicos
/
Linhagem Celular Tumoral
/
Ciclo-Oxigenase 2
/
Transição Epitelial-Mesenquimal
/
Celecoxib
Limite:
Female
/
Humans
Idioma:
En
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2020
Tipo de documento:
Article