Quantitation of Hepatitis C Virus RNA by Competitive RT-PCR and DNA-ELISA Method / 대한간학회지
The Korean Journal of Hepatology
;
: 156-171, 2000.
Artigo
em Coreano
| WPRIM
| ID: wpr-101095
ABSTRACT
BACKGROUND/AIMS:
Quantitation of Hepatitis C Virus (HCV) RNA in serum is important for monitoring the response to interferon-alpha therapy in patients with chronic hepatitis C. Several commercial assays are recently available, but they are expensive and cannot be used as a gold standard.METHODS:
An in-house competitive reverse transcription-polymerase chain reaction (cRT-PCR) was developed and validated. The procedure involves the construction of a mutant and wild type HCV RNA internal standard (IS), cRT-PCR, and colorimetric detection with DNA-ELISA. A standard curve was obtained and used for final HCV RNA quantitation.RESULTS:
The standard curve was linear over the range of 1x104 to 5x107 copies/mL of the HCV RNA standard (r=0.976). This in-house cRT-PCR was comparable with the branched DNA (bDNA) assay (Quantiplex HCV 2.0, Chiron, USA) with positive correlation between the two tests (r=0.735).CONCLUSION:
The quantitation of HCV RNA by in-house cRT-PCR and DNA ELISA was more sensitive and had wider range of detection compared to bDNA assay. This assay is useful for follow-up of HCV RNA concentration after interferon-alpha therapy.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
RNA
/
Ensaio de Imunoadsorção Enzimática
/
Interferon-alfa
/
Hepatite C
/
Hepacivirus
/
Hepatite C Crônica
/
Ensaio de Amplificação de Sinal de DNA Ramificado
/
Hepatite
Limite:
Humanos
Idioma:
Coreano
Revista:
The Korean Journal of Hepatology
Ano de publicação:
2000
Tipo de documento:
Artigo
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