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Tu-Xian Decoction ameliorates diabetic cognitive impairment by inhibiting DAPK-1 / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.) ; (6): 950-960, 2023.
Artigo em Inglês | WPRIM | ID: wpr-1011006
ABSTRACT
Tu-Xian decoction (TXD), a traditional Chinese medicine (TCM) formula, has been frequently administered to manage diabetic cognitive impairment (DCI). Despite its widespread use, the mechanisms underlying TXD's protective effects on DCI have yet to be fully elucidated. As a significant regulator in neurodegenerative conditions, death-associated protein kinase-1 (DAPK-1) serves as a focus for understanding the action of TXD. This study was designed to whether TXD mediates its beneficial outcomes by inhibiting DAPK-1. To this end, a diabetic model was established using Sprague-Dawley (SD) rats through a high-fat, high-sugar (HFHS) diet regimen, followed by streptozotocin (STZ) injection. The experimental cohort was stratified into six groups Control, Diabetic, TC-DAPK6, high-dose TXD, medium-dose TXD, and low-dose TXD groups. Following a 12-week treatment period, various assessments-including blood glucose levels, body weight measurements, Morris water maze (MWM) testing for cognitive function, brain magnetic resonance imaging (MRI), and histological analyses using hematoxylin-eosin (H&E), and Nissl staining-were conducted. Protein expression in the hippocampus was quantified through Western blotting analysis. The results revealed that TXD significantly improved spatial learning and memory abilities, and preserved hippocampal structure in diabetic rats. Importantly, TXD administration led to a down-regulation of proteins indicative of neurological damage and suppressed DAPK-1 activity within the hippocampal region. These results underscore TXD's potential in mitigating DCIvia DAPK-1 inhibition, positioning it as a viable therapeutic candidate for addressing this condition. Further investigation into TXD's molecular mechanisms may elucidate new pathways for the treatment of DCI.
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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Encéfalo / Ratos Sprague-Dawley / Diabetes Mellitus Experimental / Disfunção Cognitiva / Hipocampo Limite: Animais Idioma: Inglês Revista: Chinese Journal of Natural Medicines (English Ed.) Ano de publicação: 2023 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Encéfalo / Ratos Sprague-Dawley / Diabetes Mellitus Experimental / Disfunção Cognitiva / Hipocampo Limite: Animais Idioma: Inglês Revista: Chinese Journal of Natural Medicines (English Ed.) Ano de publicação: 2023 Tipo de documento: Artigo