Risk factors for bone loss in ulcerative colitis patients and their relationship with fecal microbiota / 西安交通大学学报(医学版)

ABSTRACT

【Objective】 To study the incidence and risk factors of bone loss in ulcerative colitis （UC） patients and understand the characteristics of fecal microbiota so as to provide new ideas for the mechanism and new treatment of bone loss in UC patients. 【Methods】 We chose adult UC patients from Department of Gastroenterology in The First Affiliated Hospital of Xi’an Jiaotong University as research subjects. We collected the patients’ related data and divided them into two groups. Then we analyzed the data to understand the incidence of and risk factors for bone loss in the UC patients. Furthermore， we collected the feces of the patients. Based on high-throughput sequencing technology， we analyzed the fecal microbiota’s diversity， species composition and metabolic pathways to understand the differences between the two groups. 【Results】 In the 24 UC patients， the incidence of bone loss was 62.5%. The incidence of bone loss was significantly higher in chronic relapsing UC patients than in initial patients， and the incidence of bone loss increased with the severity of UC. Serum calcium and 25-hydroxyvitamin D concentrations in the normal group were significantly higher than those in the bone loss group. β diversity significantly differed and the species composition of fecal microbiota significantly differed at the levels of phylum， class， order， family and genus between the two groups. Compared with the normal group， the expression abundance of Shigellosis， Geraniol degradation， Steroid hormone biosynthesis pathways was significantly increased in the bone loss group. 【Conclusion】 There is a high incidence of bone loss in UC patients. The clinical type， disease severity， serum calcium and 25-hydroxyvitamin D concentrations are related to bone loss in these patients. In the bone loss group， the diversity of fecal microbiota， especially the proportion of probiotics， is reduced， and the abundance of many disease-related metabolic pathways is significantly upregulated.