miR-223 regulates the proliferation and invasion of cervical cancer cells by targeting the ARMCX1 gene / 西安交通大学学报(医学版)

ABSTRACT

【Objective】 To investigate the effect of microRNA （miRNA， miR）-223 on the proliferation and invasion of siha cells in cervical cancer and its mechanism. 【Methods】 Fluorescent quantitative polymerase chain reaction （Real-time PCR） was used to detect the expression levels of miR-223 and arm duplication protein 1 （ARMCX1） in cervical cancer tissues. Siha cell lines overexpressed by miR-223 were established， and the effects of overexpression of miR-223 on biological functions of siha cells were tested by CCK8 and Transwell test. Wild-type and mutant ARMCX1 double luciferase reporter vectors were constructed and luciferase activity was detected. The mRNA and protein expression levels of ARMCX1 were detected by Real-time PCR and Western blotting after overexpression of miR-223 in siha cells. 【Results】 Compared with that of adjacent tissues， the expression level of miR-223 in cervical cancer tissues was significantly decreased （P<0.05）， and the mRNA expression lof ARMCX1 was significantly increased （P<0.05）. Compared with the NC mimic group， the expression of miR-223 in miR-223 mimic group was significantly increased （P<0.05）， and the cell proliferation and invasion ability were significantly reduced （P<005）. However， after transfection with GV230-ARMCX1， cell proliferation and invasion abilities were enhanced in miR-223+GV230-ARMCX group compared with miR-223+GV230-NC group （P<0.05）. The double luciferase reporting experiment confirmed that miR-223 could directly bind with ARMCX1’s 3’UTR region sequence， and Real-time PCR and Western blotting results showed that the expressions of ARMCX1 mRNA and protein were decreased （P<0.05）. 【Conclusion】 miR-223 is underexpressed in cervical cancer and can inhibit the proliferation and invasion of cervical cancer cells by targeting ARMCX1 gene.