Mechanism and main active ingredients of Radix Et Rhizoma Tripterygii Wilfordii for breast cancer / 中国药理学通报

ABSTRACT

Aim To explore the main active ingredients of Tripterygium wilfordii and the mechanism in treat-ment of breast cancer based on network pharmacology.Methods The active components and targets of Tripterygii Wilfordii were searched by TCMSP.GeneCard database was used to screen the potential targets of Tripterygii Wilfordii in treatment of breast cancer.The two were matched to obtain the core components and targets of Tripterygii Wilfordii.Cytoscape3.6.0 and AutoDock Vina were used to draw the drug-target network diagram, and GO enrichment analysis, KEGG pathway enrichment analysis, and molecular docking between the core target and the components were carried out.CCK8 and qPCR were used to verify the effect of optimal core component tripterine on breast cancer cells.Results Seven kinds of active anti-breast cancer components and twenty-five core therapeutic targets of Tripterygii Wilfordii were obtained.Tripterine, which significantly inhibited the growth of breast cancer cells, was the most valuable component of Tripterygii Wilfordii for breast cancer.QPCR results showed that tripterine decreased the expression of core therapeutic targets.Conclusions The effectiveness of Tripterygii Wilfordii has multiple pathways, and tripterine may play an important role in treatment of breast cancer.