Fluorescence PCR Amplification Related Technology / 中国生物化学与分子生物学报

ABSTRACT

Using fluorescence PCR (FPCR) technology to amplify DNA is an important part of modern biological research. The paper traced the invention of FPCR, through its main development, respective principles, design techniques, through to practical applications, etc. The two generations of phased methods of real-time quantitative PCR (QPCR) and digital PCR (DPCR) were mainly reviewed. QPCR that contained means of dyes, hydrolysis probe and its derivatives, hybridization probe containing molecular beacon and Yin-Yang probes, etc, dye melting curve and probe melting curve was summarized. DPCR involving chip digital PCR(cdPCR) and droplet digital PCR (ddPCR) was also included. Furthermore, the main application areas and limitation of FPCR, their characteristics of different types and future development direction were described.