Effect of Autophagy Inhibitor 3-MA on Intestinal Mucosal Barrier Function in Rats With Experimental Severe Acute Pancreatitis / 胃肠病学

ABSTRACT

Background: Intestinal mucosal barrier function is closely related to the prognosis of patients with severe acute pancreatitis (SAP). Protecting intestinal mucosal barrier function has become the key of treatment of SAP. Aims: To explore the relationship between autophagy and intestinal mucosal barrier function in SAP and the effect of autophagy inhibitor 3-MA. Methods: SD rats were randomly divided into sham operation (SO) group, acute necrotizing pancreatitis (ANP) group and 3-MA group. Each group was further divided into 3 subgroups 3, 6 and 12 hours after surgery. HE staining was used to observe the pathological changes of pancreatic and intestinal tissue. Blood amylase was detected; serum endotoxin and intestinal fatty acid-binding protein (I-FABP) were determined by ELISA; protein expression of Beclin-1 was examined by Western blotting. Results: Compared with SO group, pathological score of pancreatic and intestinal tissue, blood amylase, endotoxin and I-FABP were significantly increased at each time points in ANP group (P<0.05), while protein expression of Beclin-1 was significantly decreased (P<0.05), and all above-mentioned indices were time-dependent (P<0.05). After treatment with 3-MA, above-mentioned indices were significantly ameliorated (P<0.05). Conclusions: In SAP, intestinal autophagy gradually increases with the progress of disease. After intervention with 3-MA, intestinal autophagy decreases and damage of intestinal mucosal barrier function is alleviated, suggesting that intestinal autophagy is related to the damage of intestinal mucosal barrier function of SAP, and 3-MA has an inhibitory effect.