The effect of IL-22 on the expression of NLRP3 and caspase-1 mRNA and secretion of IL-18 and IL-1β in macrophages induced by lipopolysaccharide / 中华急诊医学杂志

ABSTRACT

Objective:To explore the effect of interleukin (IL)-22 on the expression of nucleotide binding oligomerization domain like receptor protein 3 (NLRP3) and caspase-1 mRNA and secretion of IL-18 and IL-1β in macrophages induced by lipopolysaccharide (LPS), RAW264.7 macrophages were cultured in vitro.Methods:Macrophage RAW264.7 was cultured in vitro, and the cultured cells were divided into three groups (control group, LPS group and LPS+IL-22 group), and the experimental cells in each group were intervened, and cultured for 3, 6 and 24 h respectively, and the cells and supernatants in each group were collected. RT-PCR, Western Blot and ELISA were used to detect NLRP3 and caspase-1 when the inflammatory body of macrophage NLRP3 was activated.Results:The expression levels of NLRP3 and caspase-1 mRNA and the secretion levels of IL-1β and IL-18 were increased in the LPS group, and the differences were statistically significant compared with the control group. After LPS and IL-22 co-stimulated macrophages, the expression levels of NLRP3 and caspase-1 mRNA, and the secretion levels of IL-1β and IL-18 were increased to different degrees, which were significantly increased compared with the LPS group.Conclusion:IL-22 could provide a new therapeutic idea for sepsis by enhancing the expression of NLRP3 and caspase-1 mRNA and the secretion of IL-18 and IL-1β in macrophages induced by LPS.