The expression and prognostic value of vascular endothelial growth factor receptor-2 and microRNA-378 in cervical cancer patients / 中国医师进修杂志

ABSTRACT

Objective:To investigate the expression and prognostic value of vascular endothelial growth factor receptor-2 (VEGFR-2) and microRNA-378 (miR-378) in cervical cancer patients.Methods:A total of 105 patients with cervical cancer diagnosed and treated in Shaoxing Central Hospital from September 2019 to September 2021 were selected. Cervical cancer tissues and adjacent normal tissues (at least 2 cm distant from the tumor tissue) were collected, respectively. Immunohistochemistry was used to detect the expression of VEGFR-2, and reverse transcription-polymerase chain reaction(RT-PCR) method was used to detect the expression of miR-378 in tissues. The positive expression rate of VEGFR-2 and the expression of miR-378 in cervical cancer tissues and adjacent normal tissues were compared, and the relationship of VEGFR-2 and miR-378 with pathological parameters and prognosis of cervical cancer was analyzed. Kaplan-Meier method was used to plot the survival curve, and univariate and multivariate Cox regression analysis was used to analyze the risk factors affecting the prognosis of patients with cervical cancer.Results:The positive expression rate of VEGFR-2 in the cervical cancer tissues was higher than that in the adjacent normal tissues: 86.67% (91/105) vs. 62.86%(66/105); the expression level of miR-378 in the cervical cancer tissues was higher than that in the adjacent normal tissues: 1.46 ± 0.35 vs. 0.68 ± 0.12, there were statistical differences ( χ2 = 15.77, t = 21.60, P<0.05). The positive expression rate of VEGFR-2 and the expression level of miR-378 in cervical cancer tissues had no relationship with age, pathological type and tumor size ( P>0.05), but with the increase of clinical stage, the decrease of differentiation and the occurrence of lymph node metastasis, the positive expression rate of VEGFR-2 and the expression level of miR-378 increased ( P<0.05). Survival curve was drawn by Kaplan-Meier method, the results showed that the overall survival time (OS) of patients with miR-378 high expression was significantly lower than patients with miR-378 low expression, and the OS of patients with VEGFR-2 positive expression was significantly lower than patients with VEGFR-2 negative expression, there were statistical differences ( P<0.05). Univariate and multivariate Cox regression analysis confirmed that clinical stage Ⅲ-Ⅳ, low differentiation, lymph node metastasis, positive expression of VEGFR-2 and high expression of miR-378 were independent risk factors for poor prognosis of cervical cancer patients ( P<0.05). Conclusions:The positive expression of VEFGR-2 and high expression of miR-378 in cervical cancer tissues are related to clinical stage, differentiation degree and lymph node metastasis. At the same time, the positive expression of VEGFR-2 and high expression of miR-378 will affect the survival of patients with cervical cancer, which is a risk factor for the poor prognosis of cervical cancer.