Effects of Yishen Ruanjian Powder on endometrial vascular remodeling in a mouse model of dysfunctional uterine bleeding / 中成药

ABSTRACT

AIM To explore the effects of Yishen Ruanjian Powder on endometrial vascular remodeling in a mouse model of dysfunctional uterine bleeding(DUB).METHODS Forty female pregnant mice were randomly divided into the control group,the model group,Gongxuening Capsule group and Yishen Ruanjian Powder group,with 10 mice in each group.The mouse models were given mifepristone(8.5 mg/kg)and misoprostol(100 μg/kg)by gavage to prepare DUB mouse model,in contrast to those of the control group given the same volume of normal saline.On the first day after successful modeling,the control group and model group were given normal saline,and the other two groups went on with their 7-day regime of Gongxuening Capsule(0.07 g/kg)and Yishen Ruanjian Powder decoction(28.9 g/kg),respectively.After the administration,the mice had their endometrial pathological changes observed by HE staining;their endometrial expression of α-SMA protein detected by immunohistochemistry;their endometrial activities of Ang Ⅱ and NO,and serum levels of FSH,LH,E2 and P hormones detected by ELISA;their endometrial protein expressions of VEGF,bFGF,MMP-9 and TGF-β detected by Western blot;and theirendometrial apoptosis detected by TUNEL.RESULTS Compared with the control group,the model group displayed disordered arrangement in the endometrial epithelial cells;thinner endometrium thickness(P<0.01);decreased endometrial NO activity and the expressions of α-SMA and VEGF(P<0.01);increased Ang Ⅱ activity,apoptosis rate and protein expressions of bFGF,MMP-9 and TGF-β(P<0.01);increased serum levels of E2,FSH,and P hormones(P<0.01);and decreased LH level(P<0.01).Compared with the model group,Yishen Ruanjian Powder and Gongxuening Capsule groups displayed superior efficacy in terms of the improvements in all aforementioned incices levels(P<0.05,P<0.01).CONCLUSION Yishen Ruanjian Powder may improve endometrial vascular remodeling disorder in mouse models of DUB by regulating the level of vasoactive substances and the expression of growth factors.