Study on isolation of Prevotella nigrescens 9336-specific DNA probes using random cloning method / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 269-279, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-103314
ABSTRACT
The purpose of this study is to develop species-specific DNA probes and polymerase chain reaction (PCR) primers for detection and identification of Prevotella nigrescens (P. nigrescens) 9336. This study procedure includes (1) whole-genomic DNA extraction of P. nigrescens 9336 (2) construction of the genomic DNA library, (3) screening of strain-specific DNA probe by reverse dot Hybridization method, (4) confirmation of strain-specific DNA probe by Southern blot analysis, (5) determination of nucleotide sequences of strain-specific DNA probe. Thirty-five restriction fragments of P. nigrescens 9336 genomic DNA digested with the Hind III were obtained. Reverse dot hybridization and Southern blot analysis data showed that three of them, Pn10, Pn23, and Pn35, could be P. nigrescens 9336-specific DNA probes. These data indicated that these DNA probes could be useful in detection and identification of the P. nigrescens 9336.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Sequência de Bases
/
Sondas de DNA
/
Biblioteca Gênica
/
Programas de Rastreamento
/
Southern Blotting
/
Reação em Cadeia da Polimerase
/
Células Clonais
/
Prevotella
/
Clonagem de Organismos
Tipo de estudo:
Ensaio Clínico Controlado
/
Estudo de rastreamento
Idioma:
Coreano
Revista:
The Journal of the Korean Academy of Periodontology
Ano de publicação:
2002
Tipo de documento:
Artigo
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