The Effect of Brimonidine on Transepithelial Resistance in a Human Retinal Pigment Epithelial Cell Line
Korean Journal of Ophthalmology
;
: 169-172, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-103548
ABSTRACT
PURPOSE:
To investigate the effects of brimonidine, an alpha-2-adrenergic agonist, on barrier function in ARPE-19 cells by measuring transepithelial resistance (TER).METHODS:
ARPE-19 cells were cultured into a confluent monolayer on a microporous filter. Brimonidine was added to the apical medium, and the barrier function of the cells was evaluated by measuring TER. A subset of cells was treated under hypoxic conditions, and the TER changes observed upon administration of brimonidine were compared to those observed in cells in normoxic conditions.RESULTS:
The ARPE cell membrane reached a peak resistance of 29.1+/-7.97 Omega cm2 after four weeks of culture. The TER of the cells treated under normoxic conditions increased with brimonidine treatment; however, the TER of the cells treated under hypoxic conditions did not change following the administration of brimonidine.CONCLUSIONS:
Barrier function in ARPE-19 cells increased with brimonidine treatment. Understanding the exact mechanism of this barrier function change requires further investigation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Quinoxalinas
/
Hipóxia Celular
/
Linhagem Celular
/
Impedância Elétrica
/
Receptores Adrenérgicos alfa 2
/
Agonistas alfa-Adrenérgicos
/
Epitélio Pigmentado da Retina
Limite:
Humanos
Idioma:
Inglês
Revista:
Korean Journal of Ophthalmology
Ano de publicação:
2010
Tipo de documento:
Artigo
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