Changes in renal cell glycolysis and amino acid metabolism during cisplatin⁃induced acute kidney injury / 安徽医科大学学报

ABSTRACT

Objective @#To evaluate the change of energy metabolism during cisplatin ⁃induced acute kidney injury.@*Methods @#Adult CD⁃1 male mice were intraperitoneally inj ected with a single dose of cisplatin (20 mg/kg) , and renal function and renal tissue pathology were tested; gene expression was analyzed and signaling pathways were enriched in cisplatin ⁃treated renal tubular epithelial cells using transcriptome; the contents of renal glycolysis and amino acid metabolites were analyzed using liquid chromatography⁃tandem mass spectrometry ( LC⁃MS/MS) . @*Results@#Serum urea nitrogen and blood creatinine significantly increased in cisplatin ⁃treated mice. Pathological histology ob served swelling and shedding of renal tubular epithelial cells. Transcriptome analysis revealed that 2 632 genes were upregulated and 2 799 genes were downregulated in cisplatin ⁃treated HK⁃2 cells. GO and KEGG analyatin caused an upregulation of the oxidative phosphorylation pathway and a downregulation of the glycolysis pathway in renal tubular epithelial cells , further KEGG analysis demonstrated that cisplatin caused changes in the expression of amino acid genes in renal cells. Metabolomics showed that the contents of glycolytic intermediates and several amino acids were altered in the kidney of cisplatin ⁃treated mice. @*Conclusion @#Cisplatin ⁃induced acute renal injury is accompanied by modification in renal tubular cell glycolysis and amino acid metabolism.