To investigate the effects of electroacupuncture and bifidobacterium on intestinal signaling substances and lipid metabolism in rats with simple obesity based on brain⁃gut axis / 安徽医科大学学报

ABSTRACT

Objective @#To observe the effects of electroacupuncture and bifidobacterium on the survival state , the level of intestinal signaling substances and lipid metabolism levels in rats with simple obesity , and to compare their efficacy. @*Methods @#Sixty 4 ⁃week⁃old SPF SD rats with the body weight ranging from 40 to 60 g were purchased , 10 (half male and half female) were given ordinary diet as blank group , and 50 (half male and half female) were given high fat diet to prepare simple obese rat model. After 8 weeks , 30 obese rats successfully established in high fat group were randomly divided into obesity model group , electroacupuncture group and bifidus group , with 10 rats in each group (half male and half female) . The treatment group was treated with electroacupuncture and bifidobacterium solution for 4 weeks , respectively , and the body mass of the rats was measured at a fixed time during the experiment. After the treatment cycle , the level of enterochromaffin cell(ECC) in colon was detected by silver staining , the levels of 5 ⁃hydroxytryptamine(5 ⁃HT) , histamine(HIS) in obese rats were detected by ELISA , the levels of to⁃ tal cholesterol(TC) , triglyceride( TG) , low density lipoprotein( LDL) and high density lipoprotein ( HDL) were detected by biochemical method. @*Results @#After intervention , compared with model group , body weight , colon and hypothalamus 5 ⁃HT , HIS , colon ECC , serum TC , TG and LDL levels decreased in electroacupuncture group and bifidobacterium group , with statistical significance (P < 0. 05) , although the serum HDL levels were increased , the difference was not statistically significant. And the indexes of the electroacupuncture group were more similar to those of the blank group than those of the bifidobacterium group. @*Conclusion @#The effect of electrotherapy on simple obesity rats is better than bifidobacterium. Electroacupuncture may stimulate the hypothalamus to reduce food intake , accelerate lipid metabolism and slow down obesity by adjusting the intestinal signaling substances in rats with simple obesity.