The protective effect of 2-BFI on SH-SY5Y cell neurotoxicity induced by NaF / 安徽医科大学学报

ABSTRACT

Objective @#To observe the effect of sodium fluoride ( NaF) and 2-(2-benzofuranyl) 2-imidazoline (2- BFI) on mitochondrial morphology，function，and apoptosis of SH-SY5Y cells，and to explore the protective effect of 2-BFI on NaF-induced injury．@*Methods @#Different concentrations of NaF (0. 5，1. 0，1. 5，2. 0，4. 0 mmol / L)were used to treat SH-SY5Y cells for 24 h，and then Cell Counting Kit-8 ( CCK-8) assay was used to screen the proliferation ability of SH-SY5Y cells ; SH-SY5Y cells were treated with NaF and different concentrations of 2-BFI (2. 5，5. 0，10. 0，20. 0，40. 0 μmol / L) for 24 h，and the proliferation ability of SH-SY5Y cells was detected by CCK-8 assay．The transmission electron microscope was used to observe the mitochondrial morphology，the mito- chondrial function was detected by ATP detection kit and Mito-Tracker Red CMXRos kit，cell apoptosis was detec- ted by TUNEL staining，and the expressions of Bcl-2，Bax，and cleaved caspase-3 protein were detected by West- ern blot. @*Results @#The cell survival rate of SH-SY5Y gradually decreased with the increase of NaF concentration. The selected NaF was 2. 0 mmol / L．2-BFI treatment for 24 h could improve the cell survival rate．At 10 mmol / L， the cell survival rate was higher (99. 169% ) ，and 10 mmol / L was selected as the 2-BFI follow-up experimental concentration．Compared with the control group，the mitochondrial morphology of the NaF group changed，ATP lev- els and mitochondrial membrane potential ( Δψm) decreased，the apoptosis rate increased，Bcl-2 protein content decreased，expression of Bax and cleaved caspase-3 protein increased．Compared with Group NaF，2-BFI adminis- tration improved mitochondria morphology，increased ATP levels and Δψm，reduced cell apoptosis rate，increased Bcl-2 protein content，and reduced Bax，cleaved caspase-3 protein expression．@*Conclusion @#2-BFI can improve mitochondrial morphology and function，reduce cell apoptosis，and play neuroprotective role in fluorosis SH-SY5Y cells.