A New Gene Regulated by Electroconvulsive Shock in Rat Brains / 신경정신의학
Journal of Korean Neuropsychiatric Association
;
: 928-935, 2000.
Artigo
em Coreano
| WPRIM
| ID: wpr-103924
ABSTRACT
OBJECTIVES:
This study was performed to identify genes regulated by electroconvulsive shock (ECS) and to observe the pattern of expression of genes according to different developmental stages and brain regions.METHODS:
ECS(130V, 0.5 sec) was given to male Sprague-Dawley rats with age of postnatal day 7 and 21(P7, P21 respectively). After screening genes regulated by ECS with mRNA differential display-PCR(DD-PCR), we selected one clone among them and observed the induction of this gene after ECS by time-dependent Northern blot analysis of rat brain of P7, P21 and adult rat cortex and hippocampus.RESULTS:
By DD-PCR method, we have identified four clones whose expression was regulated by ECS. Among them, one(CP 10-2) was proved to be a new gene by sequencing and BLAST search. Its expression was increased after ECS in P7, P21, and adult rat brain. The expression of CP 10-2 reached peak level at 180 minutes after ECS in P7 rat brain, but was further increased until 360 minutes after ECS in P21 and adult rat brain.CONCLUSION:
In this study, a new gene was identified in rat brain which showed up-regulated expression in response to ECS. Cloning and characterization of this new gene would be helpful to elucidate the effect of ECS in rat brain.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Encéfalo
/
RNA Mensageiro
/
Programas de Rastreamento
/
Northern Blotting
/
Células Clonais
/
Ratos Sprague-Dawley
/
Clonagem de Organismos
/
Eletrochoque
/
Hipocampo
Tipo de estudo:
Estudo de rastreamento
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Journal of Korean Neuropsychiatric Association
Ano de publicação:
2000
Tipo de documento:
Artigo
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