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Dot-Blot Immunoassay of Fasciola gigantica Infection using 27 kDa and Adult Worm Regurge Antigens in Egyptian Patients
The Korean Journal of Parasitology ; : 177-182, 2013.
Artigo em Inglês | WPRIM | ID: wpr-103957
ABSTRACT
The purpose of the present study was to evaluate the potential role of the 27-Kilodalton (KDa) antigen versus Fasciola gigantica adult worm regurge antigens in a DOT-Blot assay and to assess this assay as a practical tool for diagnosis fascioliasis in Egyptian patients. Fasciola gigantica antigen of an approximate molecular mass 27-(KDa) was obtained from adult worms by a simple elution SDS-PAGE. A Dot-Blot was developed comparatively to adult worm regurge antigens for the detection of specific antibodies from patients infected with F. gigantica in Egypt. Control sera were obtained from patients with other parasitic infections and healthy volunteers to assess the test and compare between the antigens. The sensitivity, specificity, positive and negative predictive values of Dot-Blot using the adult worm regurge were 80%, 90%, 94.1%, and 69.2% respectively, while those using 27-KDa were 100% which confirms the diagnostic potential of this antigen. All patients infected with Fasciola were positive, with cross reactivity reported with Schistosoma mansoni serum samples. This 27-KDa Dot-Blot assay showed to be a promising test which can be used for serodiagnosis of fascioliasis in Egyptian patients especially, those presenting with hepatic disease. It is specific, sensitive and easy to perform method for the rapid diagnosis particularly when more complex laboratory tests are unavailable.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Parasitologia / Anticorpos Anti-Helmínticos / Immunoblotting / Valor Preditivo dos Testes / Sensibilidade e Especificidade / Testes Diagnósticos de Rotina / Egito / Fasciola / Fasciolíase / Antígenos de Helmintos Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Animais / Humanos País/Região como assunto: África Idioma: Inglês Revista: The Korean Journal of Parasitology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Parasitologia / Anticorpos Anti-Helmínticos / Immunoblotting / Valor Preditivo dos Testes / Sensibilidade e Especificidade / Testes Diagnósticos de Rotina / Egito / Fasciola / Fasciolíase / Antígenos de Helmintos Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Animais / Humanos País/Região como assunto: África Idioma: Inglês Revista: The Korean Journal of Parasitology Ano de publicação: 2013 Tipo de documento: Artigo