Phylogenetic Groups and Virulence Factors of Escherichia coli Causing Urinary Tract Infection in Children
Pediatric Infection & Vaccine
; : 194-200, 2015.
Article
em Ko
| WPRIM
| ID: wpr-104102
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: Urinary tract infection (UTI) is a common bacterial infection in children and Escherichia coli is a predominant pathogen. The purpose of this study is to evaluate phylogenetic groups and virulence factors of E. coli causing UTI in children in Korea. METHODS: From October 2010 to April 2013, urinary E. coli strains were isolated from the 33 pediatric patients of UTI. Multiplex polymerase chain reactions were performed to evaluate the phylogenetic groups and 5 virulence factor genes (fimH, sfa, papA, hylA, and cnf1) of E. coli. Distribution of molecular characteristics of E. coli was analyzed by clinical diagnosis and accompanying vesicoureteral reflux (VUR). RESULTS: Most (84.8%) uropathogenic E. coli were belonged to phylogenetics group B2 and the others (15.2%) were belonged to group D. The virulence factors were distributed as: fimH (100%), sfa (100%), hylA (63.6%), cnfI (63.6%), and papA (36.4%). According to clinical diagnosis, phylogenetic distribution of E. coli strain was 92.3% of B2 and 7.7% of D in acute pyelonephritis and 57.1% of B2 and 42.9% of D in cystitis. Distribution of virulence factors was similar in both groups. In patients with acute pyelonephritis, phylogenetic distribution was similar in VUR and non-VUR group, but proportion of papA genes were lower in VUR group than that of non-VUR group (43.8% vs. 20.0%, P=0.399). CONCLUSIONS: This study provides current epidemiologic molecular data of E. coli causing pediatric UTI in Korea and will be a fundamental for understanding the pathogenesis of pediatric UTI.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Pielonefrite
/
Infecções Bacterianas
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Sistema Urinário
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Infecções Urinárias
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Refluxo Vesicoureteral
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Virulência
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Reação em Cadeia da Polimerase
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Cistite
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Fatores de Virulência
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Diagnóstico
Tipo de estudo:
Diagnostic_studies
Limite:
Child
/
Humans
País/Região como assunto:
Asia
Idioma:
Ko
Revista:
Pediatric Infection & Vaccine
Ano de publicação:
2015
Tipo de documento:
Article