Comparative analysis of cellulose acetate hemoglobin electrophoresis and high performance liquid chromatography for quantitative determination of hemoglobin A2
Blood Research
;
: 46-50, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-104393
ABSTRACT
BACKGROUND:
The present study is designed to evaluate the reliability and cost effectiveness of cellulose acetate Hb electrophoresis and high performance liquid chromatography (HPLC) in the determination of HbA2 levels.METHODS:
The test population comprised 160 individuals divided into four groups normal individuals, beta-thalassemia trait (BTT) patients, iron deficiency anemia (IDA) patients, and co-morbid patients (BTT with IDA). HbA2 levels determined using cellulose acetate Hb electrophoresis and HPLC were compared.RESULTS:
HbA2 levels were found to be diagnostic for classical BTT using either method. In co-morbid cases, both techniques failed to diagnose all cases of BTT. The sensitivity, specificity, and Youden's index for detection of the co-morbid condition was 69% and 66% for HPLC and cellulose acetate Hb electrophoresis, respectively.CONCLUSION:
This study revealed that semi-automated cellulose acetate Hb electrophoresis is more suitable for use in beta-thalassemia prevention programs in low-income countries like Pakistan. This technique is easily available, simple and cost effective.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Paquistão
/
Hemoglobina A2
/
Celulose
/
Cromatografia Líquida de Alta Pressão
/
Cromatografia Líquida
/
Sensibilidade e Especificidade
/
Análise Custo-Benefício
/
Talassemia beta
/
Anemia Ferropriva
/
Eletroforese
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Blood Research
Ano de publicação:
2015
Tipo de documento:
Artigo
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