Detection of Listeria monocytogenes in CSF from Three Patients with Meningoencephalitis by Next-Generation Sequencing
Journal of Clinical Neurology
;
: 446-451, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-104822
ABSTRACT
BACKGROUND AND PURPOSE:
Encephalitis caused by Listeria monocytogenes (L. monocytogenes) is rare but sometimes fatal. Early diagnosis is difficult using routine cerebrospinal fluid (CSF) tests, while next-generation sequencing (NGS) is increasingly being used for the detection and characterization of pathogens.METHODS:
This study set up and applied unbiased NGS to detect L. monocytogenes in CSF collected from three cases of clinically suspected listeria meningoencephalitis.RESULTS:
Three cases of patients with acute/subacute meningoencephalitis are reported. Magnetic resonance imaging and blood cultures led to a suspected diagnosis of L. monocytogenes, while the CSF cultures were negative. Unbiased NGS of CSF identified and sequenced reads corresponding to L. monocytogenes in all three cases.CONCLUSIONS:
This is the first report highlighting the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) L. monocytogenes infection. Routine application of this technology in clinical microbiology will significantly improve diagnostic methods for CNS infectious diseases.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Imageamento por Ressonância Magnética
/
Sistema Nervoso Central
/
Líquido Cefalorraquidiano
/
Doenças Transmissíveis
/
Diagnóstico Precoce
/
Diagnóstico
/
Encefalite
/
Listeria
/
Listeria monocytogenes
/
Meningite por Listeria
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Clinical Neurology
Ano de publicação:
2016
Tipo de documento:
Artigo
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