Epigallocatechin Gallate-Mediated Alteration of the MicroRNA Expression Profile in 5α-Dihydrotestosterone-Treated Human Dermal Papilla Cells
Annals of Dermatology
;
: 327-334, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-105048
ABSTRACT
BACKGROUND:
Dihydrotestosterone (DHT) induces androgenic alopecia by shortening the hair follicle growth phase, resulting in hair loss. We previously demonstrated how changes in the microRNA (miRNA) expression profile influenced DHT-mediated cell death, cell cycle arrest, cell viability, the generation of reactive oxygen species (ROS), and senescence. Protective effects against DHT have not, however, been elucidated at the genome level.OBJECTIVE:
We showed that epigallocatechin gallate (EGCG), a major component of green tea, protects DHT-induced cell death by regulating the cellular miRNA expression profile.METHODS:
We used a miRNA microarray to identify miRNA expression levels in human dermal papilla cells (DPCs). We investigated whether the miRNA expression influenced the protective effects of EGCG against DHT-induced cell death, growth arrest, intracellular ROS levels, and senescence.RESULTS:
EGCG protected against the effects of DHT by altering the miRNA expression profile in human DPCs. In addition, EGCG attenuated DHT-mediated cell death and growth arrest and decreased intracellular ROS levels and senescence. A bioinformatics analysis elucidated the relationship between the altered miRNA expression and EGCG-mediated protective effects against DHT.CONCLUSION:
Overall, our results suggest that EGCG ameliorates the negative effects of DHT by altering the miRNA expression profile in human DPCs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Di-Hidrotestosterona
/
Chá
/
Envelhecimento
/
Sobrevivência Celular
/
Genoma
/
Morte Celular
/
Espécies Reativas de Oxigênio
/
Folículo Piloso
/
Biologia Computacional
/
MicroRNAs
Limite:
Humanos
Idioma:
Inglês
Revista:
Annals of Dermatology
Ano de publicação:
2016
Tipo de documento:
Artigo
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