Proteomic Analysis between U87MG and U343MG-A Cell Lines: Searching for Candidate Proteins for Glioma Invasion
Brain Tumor Research and Treatment
;
: 22-28, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-106236
ABSTRACT
BACKGROUND:
To investigate the molecular basis for invasion of malignant gliomas, proteomic analysis approach was carried out using two human glioma cell lines, U87MG and U343MG-A that demonstrate different motility and invasiveness in in vitro experiments.METHODS:
High-resolution two-dimensional gel electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry analysis were performed.RESULTS:
Nine distinct protein spots that were recognized with significant alteration between the two cell lines. Five of these protein spots were up-regulated in U87MG and four were up-regulated in U343MG-A.CONCLUSION:
Among these proteins, cathepsin D was shown to be one of the important proteins which are related with glioma invasion. However, further studies are necessary to reveal the exact role and mechanism of cathepsin D in glioma invasion.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Espectrometria de Massas
/
Eletroforese em Gel Bidimensional
/
Linhagem Celular
/
Catepsina D
/
Proteômica
/
Glioma
Limite:
Humanos
Idioma:
Inglês
Revista:
Brain Tumor Research and Treatment
Ano de publicação:
2014
Tipo de documento:
Artigo
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