Age-Related Changes in Antioxidative Enzyme Capacity in Tongue of Fischer 344 Rats
Clinical and Experimental Otorhinolaryngology
;
: 352-357, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-106631
ABSTRACT
OBJECTIVES:
Antioxidative enzyme efficiency changes in some organs with age. However, no study has been conducted on age-related antioxidant enzyme changes in tongue. In the present study, the authors investigated the activities of four antioxidative enzymes and their protein expressions in the tongues of young and old Fischer 344 rats.METHODS:
Age-dependent changes in the enzyme activities of total superoxide dismutase (SOD), Mn-SOD, Cu/Zn-SOD, catalase (CAT), and glutathione peroxidase (GPx) were determined using chemical kits, and the protein expressions levels of these enzymes by Western blotting. The study was conducted using rats aged 7 months (the young group, n=8) and 22 months (the old group, n=8).RESULTS:
Total SOD, Cu/Zn-SOD, and GPx activities in the tongues of old rats were lower than in young rats, and similarly, corresponding protein expressions were downregulated in old rats. On the other hand, although the protein expressions of Mn-SOD and CAT were lower in old rats, their enzyme activities were not.CONCLUSION:
The results of this study provide a possible mechanism for the tongue aging process, as in old Fischer 344 rats the antioxidant defense system was diminished with respect to enzyme activity levels and protein abundances.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Superóxido Dismutase
/
Língua
/
Envelhecimento
/
Catalase
/
Western Blotting
/
Estresse Oxidativo
/
Glutationa Peroxidase
/
Mãos
/
Antioxidantes
Limite:
Animais
Idioma:
Inglês
Revista:
Clinical and Experimental Otorhinolaryngology
Ano de publicação:
2016
Tipo de documento:
Artigo
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