Implant surface treatments affect gene expression of Runx2, osteogenic key marker
The Journal of Advanced Prosthodontics
;
: 91-96, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-107295
ABSTRACT
STATEMENT OF PROBLEM:
The aim of this study was to study the effects of various surface treatments to a titanium surface on the expression of Runx2 in vitro. MATERIAL ANDMETHODS:
Human Osteosarcoma TE-85 cells were cultured on machined, sandblasted, or anodic oxidized cpTi discs. At various times of incubation, the cells were collected and then processed for the analysis of mRNA expression of Runx2 using reverse transcription-PCR.RESULTS:
The expression pattern of Runx2 mRNA was differed according to the types of surface treatment. When the cells were cultured on the untreated control culture plates, the gene expression of Runx2 was not increased during the experiments. In the case of that the cells were cultured on the machined cpTI discs, the expression level was intermediate at the first day, but increased constitutively to day 5. In cells on sandblasted cpTi discs, the expression level was highest in the first day sample and the level was maintained to 5 days. In cells on anodized cpTi discs, the expression level increased rapidly to 3 days, but decreased slightly in the 5-th day sample.CONCLUSION:
Different surface treatments may contribute to the regulation of osteoblast function by influencing the level of gene expression of key osteogenic factors.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Osteogênese
/
Titânio
/
RNA Mensageiro
/
Osteossarcoma
/
Expressão Gênica
/
Durapatita
Limite:
Humanos
Idioma:
Inglês
Revista:
The Journal of Advanced Prosthodontics
Ano de publicação:
2009
Tipo de documento:
Artigo
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