Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair
Journal of Veterinary Science
;
: 295-297, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-108422
ABSTRACT
The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doença das Mucosas por Vírus da Diarreia Viral Bovina
/
RNA Viral
/
Vírus da Diarreia Viral Bovina Tipo 1
/
Vírus da Diarreia Viral Bovina Tipo 2
/
Reação em Cadeia da Polimerase em Tempo Real
/
Cabelo
/
Anticorpos Antivirais
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2011
Tipo de documento:
Artigo
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