Comparison of IgE-binding components between 2 house dust mites in adult allergic patients
Allergy, Asthma & Respiratory Disease
;
: 199-204, 2016.
Artigo
em Coreano
| WPRIM
| ID: wpr-108724
ABSTRACT
PURPOSE:
This study investigated the differences in the profile of IgE-binding components between Dermatophagoides pteronyssinus (Dp) and Dermatophagoides farina (Df) in respiratory allergic patients sensitized to Dp/Df.METHODS:
Eighteen patients with respiratory allergic diseases having higher levels of serum specific IgE to Df compared to those to Dp (>twice) were enrolled. IgE-immunoblot analysis using Dp and Df extracts were used to compare the IgE binding components. Study subjects were classified into 2 groups according to the results of IgE-immunoblotanalysis:
6 subjects having IgE-binding components to group 1 and 2 allergens (group B) and 12 subjects not having them (group A).RESULTS:
Group A subjects were older (47.92±8.51 vs. 35.50±11.10, P=0.039) and males were dominant (75% vs. 0% P=0.009). IgE-immunoblot analysis demonstrated that all the group B subjects had IgE bindings to 2 major components, 14 and 25 kDa, while group A subjects had IgE bindings to high-molecular weight components ranging from 60-98 kDa. The enzyme-linked immunosorbent assay inhibition test showed a significant inhibition with additions of Df, not with Dp in group B subjects. Serum specific IgE levels to Dp and Df were significantly higher in group B than in group A, while its ratio (Df to Dp) was significantly higher in group A. No differences were noted in clinical parameters, total IgE, or eosinophil cationic protein levels.CONCLUSION:
Heterogeneity of IgE binding patterns to Dp and Df extracts was noted according to the ratio of serum specific IgE (Df/Dp).
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Características da População
/
Imunoglobulina E
/
Ensaio de Imunoadsorção Enzimática
/
Alérgenos
/
Pyroglyphidae
/
Dermatophagoides farinae
/
Dermatophagoides pteronyssinus
/
Proteína Catiônica de Eosinófilo
/
Poeira
Limite:
Adulto
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Allergy, Asthma & Respiratory Disease
Ano de publicação:
2016
Tipo de documento:
Artigo
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