Cloning of canine GM-CSF and SCF genes
Journal of Veterinary Science
;
: 159-166, 2001.
Artigo
em Inglês
| WPRIM
| ID: wpr-109439
ABSTRACT
Cytokines have pleiotropic regulatory effects on hematopoietic cells and many other cell types that participate in host defence and repair processes. Granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates the growth and differentiation of granulocytes and macrophages and regulates the biological functions expressed by mature cells of these lineages. Stem cell factor (SCF) is a multifunctional cytokine involved in hematopoiesis, melanogenesis and gametogenesis. In order to determine the complementary DNA (cDNA) of canine GM-CSF and canine SCF, cDNA clones were generated from lipopolysaccharide (LPS) stimulated peripheral blood mononuclear cells (PBMCs) and bone marrow cells by reverse transcription PCR amplification. The canine GM-CSF cDNA obtained in this study contains an open reading frame encoding 144 amino acid residues and has 53-75% homology with those of human, cat, sheep, pig, cow and mouse, Canine SCF cDNA consist of an open reading frame encoding 274 amino acid residues and shares 81-92% homology with those of human, cat, pig, cow and mouse.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Códon
/
Ovinos
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Amplificação de Genes
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Fases de Leitura Aberta
/
Alinhamento de Sequência
/
Sequência de Aminoácidos
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2001
Tipo de documento:
Artigo
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