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Histologic Characteristics and Mechanical Properties of Bovine Pericardium Treated with Decellularization and alpha-Galactosidase: A Comparative Study
The Korean Journal of Thoracic and Cardiovascular Surgery ; : 368-379, 2012.
Artigo em Inglês | WPRIM | ID: wpr-109678
ABSTRACT

BACKGROUND:

Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. alpha-galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with alpha-galactosidase. MATERIALS AND

METHODS:

Bovine pericardial tissues were allocated into three groups fixation only with glutaraldehyde, decellularization with sodium dodesyl sulfate and decellularization plus treatment with alpha-galactosidase. We confirmed immunohistological characteristics and mechanical properties as fatigue test, permeability test, compliance test, tensile strength (strain) test and thermal stability test.

RESULTS:

Decellularization and elimination of alpha-gal were confirmed through immunohistologic findings. Decellularization had decreased mechanical properties compared to fixation only group in permeability (before fatigue test p=0.02, after fatigue test p=0.034), compliance (after fatigue test p=0.041), and tensile strength test (p=0.00). The group of decellularization plus treatment with alpha-galactosidase had less desirable mechanical properties than the group of decellularization in concerns of permeability (before fatigue test p=0.043) and strain test (p=0.001).

CONCLUSION:

Favorable decellularization and elimination of alpha-gal were obtained in this study through immunohistologic findings. However, those treatment including decellularization and elimination of alpha-gal implied the decreased mechanical properties in specific ways. We need more study to complete appropriate ioprosthesis with decellularization and elimination of alpha-gal including favorable mechanical properties too.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Pericárdio / Permeabilidade / Sódio / Entorses e Distensões / Resistência à Tração / Bioprótese / Glutaral / Alfa-Galactosidase / Complacência (Medida de Distensibilidade) / Fadiga Idioma: Inglês Revista: The Korean Journal of Thoracic and Cardiovascular Surgery Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Pericárdio / Permeabilidade / Sódio / Entorses e Distensões / Resistência à Tração / Bioprótese / Glutaral / Alfa-Galactosidase / Complacência (Medida de Distensibilidade) / Fadiga Idioma: Inglês Revista: The Korean Journal of Thoracic and Cardiovascular Surgery Ano de publicação: 2012 Tipo de documento: Artigo