Histologic Characteristics and Mechanical Properties of Bovine Pericardium Treated with Decellularization and alpha-Galactosidase: A Comparative Study
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 368-379, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-109678
ABSTRACT
BACKGROUND:
Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. alpha-galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with alpha-galactosidase. MATERIALS ANDMETHODS:
Bovine pericardial tissues were allocated into three groups fixation only with glutaraldehyde, decellularization with sodium dodesyl sulfate and decellularization plus treatment with alpha-galactosidase. We confirmed immunohistological characteristics and mechanical properties as fatigue test, permeability test, compliance test, tensile strength (strain) test and thermal stability test.RESULTS:
Decellularization and elimination of alpha-gal were confirmed through immunohistologic findings. Decellularization had decreased mechanical properties compared to fixation only group in permeability (before fatigue test p=0.02, after fatigue test p=0.034), compliance (after fatigue test p=0.041), and tensile strength test (p=0.00). The group of decellularization plus treatment with alpha-galactosidase had less desirable mechanical properties than the group of decellularization in concerns of permeability (before fatigue test p=0.043) and strain test (p=0.001).CONCLUSION:
Favorable decellularization and elimination of alpha-gal were obtained in this study through immunohistologic findings. However, those treatment including decellularization and elimination of alpha-gal implied the decreased mechanical properties in specific ways. We need more study to complete appropriate ioprosthesis with decellularization and elimination of alpha-gal including favorable mechanical properties too.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pericárdio
/
Permeabilidade
/
Sódio
/
Entorses e Distensões
/
Resistência à Tração
/
Bioprótese
/
Glutaral
/
Alfa-Galactosidase
/
Complacência (Medida de Distensibilidade)
/
Fadiga
Idioma:
Inglês
Revista:
The Korean Journal of Thoracic and Cardiovascular Surgery
Ano de publicação:
2012
Tipo de documento:
Artigo
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