Alterations in antioxidant function and cell apoptosis in duck spleen exposed to molybdenum and/or cadmium
Journal of Veterinary Science
;
: 193-200, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-109779
ABSTRACT
To investigate the effects of molybdenum (Mo) and/or cadmium (Cd) on antioxidant function and the apoptosis-related genes in duck spleens. Sixty healthy 11-day-old ducks were randomly divided into six groups of 10 ducks (control, low Mo group, high Mo, Cd, low Mo + Cd, and high Mo + Cd groups). All were fed a basal diet containing low or high dietary doses of Mo and/or Cd. Relative spleen weight, antioxidant indices, apoptosis-related gene mRNA expression levels, and ultrastructural changes were evaluated after 120 days. The results showed that the relative spleen weight decreased significantly in the high Mo + Cd treatment group which compared with control group. Malondialdehyde levels increased and xanthine oxidase and catalase activities decreased in the Mo and/or Cd groups compared with levels in the control group. Bak-1 and Caspase-3 expressions were upregulated in the high Mo + Cd group, while Bcl-2 was downregulated. In addition, mitochondrial crest fracture, swelling, vacuolation, deformed nuclei, and karyopyknosis in both Mo + Cd treated groups were more severe than in the other groups. The results suggest that Mo and/or Cd can induce oxidative stress and apoptosis of spleen via effects on the mitochondrial intrinsic pathway. Moreover, the results indicate the two elements have a possible synergistic relationship.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Baço
/
Xantina Oxidase
/
Cádmio
/
RNA Mensageiro
/
Catalase
/
Apoptose
/
Estresse Oxidativo
/
Dieta
/
Patos
/
Caspase 3
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2017
Tipo de documento:
Artigo
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