Serial Micro-CT Assessment of the Therapeutic Effects of Rosiglitazone in a Bleomycin-Induced Lung Fibrosis Mouse Model
Korean Journal of Radiology
;
: 448-455, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-109967
ABSTRACT
OBJECTIVE:
The aim of this study was to assess the therapeutic effects of rosiglitazone with serial micro-CT findings before and after rosiglitazone administration in a lung fibrosis mouse model induced with bleomycin. MATERIALS ANDMETHODS:
We instilled the bleomycin solution directly into the trachea in twenty mice (female, C57BL/6 mice). After the instillation with bleomycin, mice were closely observed for 3 weeks and then all mice were scanned using micro-CT without sacrifice. At 3 weeks, the mice were treated with rosiglitazone on days 21 to 27 if they had abnormal CT findings (n = 9, 45%). For the mice treated with rosiglitazone, we performed micro-CT with mouse sacrifice 2 weeks after the rosiglitazone treatment completion. We assessed the abnormal CT findings (ground glass attenuation, consolidation, bronchiectasis, reticular opacity, and honeycombing) using a five-point scale at 3 and 6 weeks using Wilcoxon-signed ranked test. The micro-CT findings were correlated with the histopathologic results.RESULTS:
One out of nine (11.1%) mice improved completely. In terms of consolidation, all mice (100%) showed marked decrease from 3.1 +/- 1.4 at 3 weeks to 0.9 +/- 0.9 at 6 weeks (p = 0.006). At 6 weeks, mild bronchiectasis (n = 6, 66.7%), mild reticular opacity (n = 7, 77.8%) and mild honeycomb patterns (n = 3, 33.3%) appeared.CONCLUSION:
A serial micro-CT enables the evaluation of drug effects in a lung fibrosis mouse model.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fibrose Pulmonar
/
Bleomicina
/
Variações Dependentes do Observador
/
Tiazolidinedionas
/
Modelos Animais de Doenças
/
Microtomografia por Raio-X
/
Camundongos Endogâmicos C57BL
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Journal of Radiology
Ano de publicação:
2014
Tipo de documento:
Artigo
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