In Vivo Target RNA Specificity of Trans-Splicing Phenomena by the Group I Intron
Genomics & Informatics
;
: 84-86, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-110091
ABSTRACT
The Tetrahymena group I intron has been shown to employ a trans-splicing reaction and has been modified to specifically target and replace human telomerase reverse transcriptase (hTERT) RNA with a suicide gene transcript, resulting in the induction of selective cytotoxicity in cancer cells that express the target RNA, in animal models as well as in cell cultures. In this study, we evaluated the target RNA specificity of trans -splicing phenomena by the group I intron in mice that were intraperitoneally inoculated with hTERT-expressing human cancer cells to validate the anti-cancer therapeutic applicability of the group I intron. To this end, an adenoviral vector that encoded for the hTERT-targeting group I intron was constructed and systemically injected into the animal. 5'-end RACE-PCR and sequencing analyses of the trans-spliced cDNA clones revealed that all of the analyzed products in the tumor tissue of the virus-infected mice resulted from reactions that were generated only with the targeted hTERT RNA. This study implies the in vivo target specificity of the trans - splicing group I intron and hence suggests that RNA replacement via a trans -splicing reaction by the group I intron is a potent anti-cancer genetic approach.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suicídio
/
Tetrahymena
/
RNA
/
Íntrons
/
Sensibilidade e Especificidade
/
Células Clonais
/
DNA Complementar
/
Telomerase
/
Técnicas de Cultura de Células
/
Trans-Splicing
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Genomics & Informatics
Ano de publicação:
2008
Tipo de documento:
Artigo
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