Phenotypic Characterization of Ligamentum Flavum Cells from Patients with Ossification of Ligamentum Flavum
Yonsei Medical Journal
;
: 375-379, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-110999
ABSTRACT
PURPOSE:
The objective of this study was to determine the phenotypic characterization of ligamentum flavum cells from patients with ossification of the ligamentum flavum (OLF). MATERIALS ANDMETHODS:
Ligamentum flavum tissues were harvested from OLF and non-OLF patients during surgery. OLF and non-OLF cells were isolated from explant cultures. Cultured cells were analyzed using immunofluorescence staining and reverse transcription-polymerase chain reaction.RESULTS:
OLF cells exhibited various appearances compared with the typical fibroblast-like morphology of non-OLF cells. Expressions of collagen type I and collagen type III were observed in OLF and non-OLF cells. OLF cells uniquely expressed osteocalcin, which is a marker for osteoblasts, and collagen type II which is a marker for chondrocytes, whereas they were negative in non-OLF cells.CONCLUSION:
These findings indicate that OLF cells have phenotypic characterization of osteoblasts and chondrocytes which could play a role in the pathophysiology of OLF.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteocalcina
/
Células Cultivadas
/
Ossificação Heterotópica
/
Ligamento Amarelo
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Colágeno Tipo I
/
Colágeno Tipo II
/
Colágeno Tipo VI
/
Microscopia de Fluorescência
Limite:
Adolescente
/
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2009
Tipo de documento:
Artigo
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