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Elevated Expression of p73 and Its Relation to VEGF and TSP-1 Production in Cervical Carcinoma / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology ; : 599-610, 2006.
Artigo em Coreano | WPRIM | ID: wpr-111317
ABSTRACT

OBJECTIVE:

Measure the over-expression of p73 and analyze as the prognostic as well as angiogenic factor of cervical cancer by comparing the degree of expression of VEGF and TSP-1 by RT-PCR.

METHODS:

7 normal and 37 cervical cancer specimens were put through RT-PCR and the expression of p73, VEGF and TSP-1 were measured. After immunohistochemical staining, the number of microvessels was counted. With the level of expression, investigated the relationship with the clinicopathological characteristics and the number of microvessels.

RESULTS:

57% of cancer tissues showed abnormally high levels of p73 mRNA. In quantitative genomic DNA PCR, the p73 was over-expressed in the transcription level. Through allotyping with Sty I polymorphism, the over-expression of p73 was due to the transcription activity of the silent allele. In RT-PCR-SSCP analysis of over-expressed specimens, sequence alterations was not seen. In 73%, VEGF was over-expressed while TSP-1 was under-expressed in 35%. There was no association between the number of microvessels with the over-expression of p73 and VEGF, but inversely associated with the under-expression of TSP-1. There was no correlation between the over-expression of p73 and the clinicopathological characteristics. The over-expression of p73 coincided 80% with the over-expression of VEGF, and 40% with the under-expression of TSP-1.

CONCLUSION:

These data support the expression of p73 was increased in cervical cancer tissues and was associated with the over-expression of the VEGF but not associated with the under-expression of TSP-1. The biological and clinical significance of the over-expression of p73 should be studied further in the future.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: DNA / RNA Mensageiro / Neoplasias do Colo do Útero / Reação em Cadeia da Polimerase / Trombospondina 1 / Fator A de Crescimento do Endotélio Vascular / Indutores da Angiogênese / Alelos / Microvasos Tipo de estudo: Estudo prognóstico Idioma: Coreano Revista: Korean Journal of Obstetrics and Gynecology Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: DNA / RNA Mensageiro / Neoplasias do Colo do Útero / Reação em Cadeia da Polimerase / Trombospondina 1 / Fator A de Crescimento do Endotélio Vascular / Indutores da Angiogênese / Alelos / Microvasos Tipo de estudo: Estudo prognóstico Idioma: Coreano Revista: Korean Journal of Obstetrics and Gynecology Ano de publicação: 2006 Tipo de documento: Artigo