The Effect of Green Tea Extract on Cisplatin in Cervical Cancer Cell Lines / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 592-598, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-111318
ABSTRACT
OBJECTIVE:
Green tea polyphenol (GTP) has been shown to have anti-tumor properties in a wide variety of experimental systems. In this study, we evaluated the effects of GTP on the cytotoxic effects of cisplatin in cultured HeLa and SiHa cells.METHODS:
The cell lines from Korean Cell Culture Bank were cultured in a RPMI-1640 medium supplemented with a 10% fetal bovine serum, antibiotics streptomycin and penicillin. GTP was extracted from tea leaves (Camellia scinensis) by water extraction and organic solvent fractionation. Cells were seeded at 1 x 10(4) cells/well in RPMI1640 media in triplicate wells on a Nunc Labware 96 well flat bottom microculture plate, with and without GTP (100 microgram/mL) and at different concentrations of cisplatin (0-1000 microgram/mL). After incubating the plates at 37 degrees C in 5% CO2 for 2 days, cell viability was determined using the MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; thiazolyl blue] assay.RESULTS:
The viability of the HeLa cells was decreased to 14% at a 600 microgram/mL concentration of cisplatin, and to 16% at 600 microgram/mL in the SiHa cells as measured by the MTT assay. However, in the HeLa cell, co-cultured with GTP (100 microgram/mL), the cell viability decreased to 68% at 200 microgram/mL of cisplatin and to 17% at 400 microgram/mL of cisplatin. And in the SiHa cell, co-cultured with GTP (100 microgram/mL), the cell viability decreased to 48% at 200 microgram/mL of cisplatin and to 17% at 400 microgram/mL of cisplatin.CONCLUSION:
This study showed that cisplatin with GTP seems to have a potentiating effect on Cisplatin cytotoxicity than cisplatin alone.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Penicilinas
/
Chá
/
Água
/
Células HeLa
/
Estreptomicina
/
Linhagem Celular
/
Sobrevivência Celular
/
Neoplasias do Colo do Útero
/
Cisplatino
/
Técnicas de Cultura de Células
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2006
Tipo de documento:
Artigo
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