Effect of paternal folate deficiency on placental folate content and folate receptor alpha expression in rats
Nutrition Research and Practice
;
: 112-116, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-111817
ABSTRACT
We investigated the effect of paternal folate status on folate content and expression of the folate transporter folate receptor alpha (FRalpha) in rat placental tissues. Rats were mated after males were fed a diet containing 0 mg of folic acid/kg of diet (paternal folate-deficient, PD) or 8 mg folic acid/kg of diet (paternal folate-supplemented, PS) for 4 weeks. At 20 days of gestation, the litter size, placental weight, and fetal weight were measured, and placental folate content (n = 8/group) and expression of FRalpha (n = 10/group) were analyzed by microbiological assay and Western blot analysis, respectively. Although there was no difference observed in litter size or fetal weight, but significant reduction (10%) in the weight of the placenta was observed in the PD group compared to that in the PS group. In the PD group, placental folate content was significantly lower (by 35%), whereas FRalpha expression was higher (by 130%) compared to the PS group. Our results suggest that paternal folate status plays a critical role in regulating placental folate metabolism and transport.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Placenta
/
Western Blotting
/
Peso Fetal
/
Dieta
/
Receptor 1 de Folato
/
Ácido Fólico
/
Tamanho da Ninhada de Vivíparos
Limite:
Animais
/
Humanos
/
Masculino
/
Gravidez
Idioma:
Inglês
Revista:
Nutrition Research and Practice
Ano de publicação:
2011
Tipo de documento:
Artigo
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