Correlation Between Neuronal Apoptosis and Expression of Inducible Nitric Oxide Synthase after Transient Focal Cerebral Ischemia
Korean Journal of Pathology
;
: 364-371, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-112683
ABSTRACT
BACKGROUND:
Neuronal death in acute-phase cerebral ischemic injury is caused by necrosis. However, neuronal injury after reperfusion can be associated with apoptosis.METHODS:
We used Sprague-Dawley rats whose brains were reperfused after middle cerebral artery occlusion for either 30 min or 2 h. We examined a relationship between apoptosis and the expression of inducible nitric oxide synthase (iNOS) in the brain tissue from 3 h to 14 days after reperfusion in both groups.RESULTS:
TUNEL and iNOS positivity were closely related in both groups. The 2-h ischemia group exhibited increases in the amount of TUNEL and iNOS-positive cells for up to 3 days after reperfusion, at which the TUNEL and iNOS-positive cells decreased. The 30-min ischemia group exhibited peak positivity 24 h after reperfusion, followed by a similar decrease. iNOS mRNA expression peaked 3 h after reperfusion in the 30-min ischemia group, at which time it decreased. In the 2-h ischemia group, iNOS mRNA increased 3 h after reperfusion, peaked 24 h after reperfusion, and then decreased.CONCLUSION:
These results indicated the occurrence of delayed apoptosis in transient cerebral ischemia. Increased expression of iNOS is closely associated with this apoptosis, and oxygen free radical-producing materials, such as nitric oxide, may play an important role in the induction of this apoptosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oxigênio
/
Encéfalo
/
RNA Mensageiro
/
Reperfusão
/
Isquemia Encefálica
/
Ataque Isquêmico Transitório
/
Ratos Sprague-Dawley
/
Apoptose
/
Marcação In Situ das Extremidades Cortadas
/
Infarto da Artéria Cerebral Média
Idioma:
Inglês
Revista:
Korean Journal of Pathology
Ano de publicação:
2004
Tipo de documento:
Artigo
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