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Correlation Between Neuronal Apoptosis and Expression of Inducible Nitric Oxide Synthase after Transient Focal Cerebral Ischemia
Korean Journal of Pathology ; : 364-371, 2004.
Artigo em Inglês | WPRIM | ID: wpr-112683
ABSTRACT

BACKGROUND:

Neuronal death in acute-phase cerebral ischemic injury is caused by necrosis. However, neuronal injury after reperfusion can be associated with apoptosis.

METHODS:

We used Sprague-Dawley rats whose brains were reperfused after middle cerebral artery occlusion for either 30 min or 2 h. We examined a relationship between apoptosis and the expression of inducible nitric oxide synthase (iNOS) in the brain tissue from 3 h to 14 days after reperfusion in both groups.

RESULTS:

TUNEL and iNOS positivity were closely related in both groups. The 2-h ischemia group exhibited increases in the amount of TUNEL and iNOS-positive cells for up to 3 days after reperfusion, at which the TUNEL and iNOS-positive cells decreased. The 30-min ischemia group exhibited peak positivity 24 h after reperfusion, followed by a similar decrease. iNOS mRNA expression peaked 3 h after reperfusion in the 30-min ischemia group, at which time it decreased. In the 2-h ischemia group, iNOS mRNA increased 3 h after reperfusion, peaked 24 h after reperfusion, and then decreased.

CONCLUSION:

These results indicated the occurrence of delayed apoptosis in transient cerebral ischemia. Increased expression of iNOS is closely associated with this apoptosis, and oxygen free radical-producing materials, such as nitric oxide, may play an important role in the induction of this apoptosis.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oxigênio / Encéfalo / RNA Mensageiro / Reperfusão / Isquemia Encefálica / Ataque Isquêmico Transitório / Ratos Sprague-Dawley / Apoptose / Marcação In Situ das Extremidades Cortadas / Infarto da Artéria Cerebral Média Idioma: Inglês Revista: Korean Journal of Pathology Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oxigênio / Encéfalo / RNA Mensageiro / Reperfusão / Isquemia Encefálica / Ataque Isquêmico Transitório / Ratos Sprague-Dawley / Apoptose / Marcação In Situ das Extremidades Cortadas / Infarto da Artéria Cerebral Média Idioma: Inglês Revista: Korean Journal of Pathology Ano de publicação: 2004 Tipo de documento: Artigo