Characteristics of Mitochondrial Events in Synthetic Bile Acids-induced Apoptosis of Human Osteosarcoma Cells / 체질인류학회지
Korean Journal of Physical Anthropology
;
: 351-362, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-113425
ABSTRACT
ABSTRACT Apoptosis of osteosarcoma cells induced by bile duct derivates, HS-1200 was investigated with relation to mitochodria. HS-1200 induced cytochrome c and Smac/DIABLO release from mitochondria which are major factors related to apoptosis. In these apoptosis processes, release of cytochrome c was not blocked by caspase inhibitor, but release of Smac/DIABLO was blocked. BKA, a kind of PTP (permeablity transition pore) inhibitor, did not block both of them. Interestingly, the alteration of MMP was not observed by means of using JC-1 dye. Although MitoTracker, DiOC-6 and Rhodamine123 were used to confirm previous results, the decrease of MMP was not observed. In order to investigate whether this phenomenon is apoptosis-specific or cell-specific process, genistein was added to cells which usually decreased MMP. After adding genistein, MMP was not decreased, suggesting this phenomenon is cell-specific process. Conclusionally, HS-1200 induced apoptosis of osteosarcoma cells via mitochondria, cytochrome c and Smac/DIABLO were released from mitochondria without decrease of MMP. The release of Smac/DIABLO was dependent of caspase.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bile
/
Ductos Biliares
/
Osteossarcoma
/
Apoptose
/
Genisteína
/
Citocromos c
/
Mitocôndrias
Limite:
Humanos
Idioma:
Inglês
Revista:
Korean Journal of Physical Anthropology
Ano de publicação:
2004
Tipo de documento:
Artigo
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