Loss of infectivity of Neospora caninum oocysts maintained for a prolonged time
The Korean Journal of Parasitology
;
: 295-299, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-114842
ABSTRACT
The purpose of this study was to investigate whether sporulated Neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4 degrees C, remain morphologically viable and infective to gerbils (Meriones unguiculatus). Six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. Two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for N. caninum at 1 50 in an immunofluorescent antibody test. PCR using the brain from each gerbil did not reveal N. caninum specific DNA. We conclude that oocysts preserved for 46 mo are not infective, despite being morphologically intact.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Refrigeração
/
Ácidos
/
Virulência
/
Encéfalo
/
Bovinos
/
Reação em Cadeia da Polimerase
/
Gerbillinae
/
Coccidiose
/
Neospora
/
Oocistos
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2007
Tipo de documento:
Artigo
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