Identification of Brucella melitensis isolates originating from Mongolia and diagnostic real-time PCR evaluation using a specific SNP / 대한수의학회지
Korean Journal of Veterinary Research
;
: 105-110, 2015.
Artigo
em Coreano
| WPRIM
| ID: wpr-114946
ABSTRACT
A real-time PCR assay using hybridization probe (HybProbe) has been developed to detect Brucella (B.) melitensis strains. The primer and HybProbe sets were designed based on the gap gene of chromosome I with a specific single nucleotide polymorphism of B. melitensis. Specificity of the assay was confirmed by comparison to reference Brucella species and other related strains. In the melting curve analysis, B. melitensis generated a peak at 67degrees C unlike those for other Brucella species observed at 61degrees C. Sensitivity of the assay for B. melitensis ranged from 20 ng to 200 fg of genomic DNA. The ability to identify 94 Mongolian B. melitensis isolates using the real-time PCR assay was identical to that of classical biotyping methods and differential multiplex PCR. These data showed that this new molecular technique is a simple and quick method for detecting B. melitensis, which will be important for the control and prevention of brucellosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Brucella
/
Brucelose
/
DNA
/
Sensibilidade e Especificidade
/
Brucella melitensis
/
Polimorfismo de Nucleotídeo Único
/
Reação em Cadeia da Polimerase Multiplex
/
Reação em Cadeia da Polimerase em Tempo Real
/
Congelamento
/
Mongólia
Tipo de estudo:
Estudo diagnóstico
País/Região como assunto:
Ásia
Idioma:
Coreano
Revista:
Korean Journal of Veterinary Research
Ano de publicação:
2015
Tipo de documento:
Artigo
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