Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
Journal of Veterinary Science
;
: 333-341, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-115774
ABSTRACT
The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virulência
/
Genoma
/
Análise de Sequência
/
Sequências de Repetição em Tandem
/
Genômica
/
Tipagem Molecular
/
Coreia (Geográfico)
/
Mycobacterium
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2017
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS