Pulsed-Field Gel Electrophoresis Pattern, Phage Type and Drug Susceptibility of Salmonella typhi Isolates from an Outbreak in Pusan City in 1996 / 감염
Korean Journal of Infectious Diseases
;
: 201-208, 1997.
Artigo
em Coreano
| WPRIM
| ID: wpr-116635
ABSTRACT
BACKGROUND:
During the period from April to June 1996, an outbreak of typhoid fever occurred in SuYoung-Ku, Pusan city. We performed the molecular epidemiological analysis and drug susceptibility test for 48 isolates of Salmonella typhi from the outbreak.METHODS:
Chromosomal DNA of S. typhi was digested with the restriction endonuclease Spe I and the resulting restriction polymorphism was observed on pulsed field gel electrophoresis (PFGE). Phage typing was performed using Vi-phages, and antimicrobial susceptibility test was done by Disc diffusion method.RESULTS:
On phage typing, 2 isolates were M1 phage type, 2 E1 phage type and 44 untypable. The PFGE analysis on 14 to 16 chromosomal DNA restriction fragments ranged from 50 Kb to 1000kb showed 2 different patterns of restriction fragments, divided into group A and B. Forty-seven isolates belonged to group A that were allocated into A1 (42 strains), A2 (1 strain), A3 (3 strains), A4 (1 strain), and one strain was grouped into group B. In the F-value of genetic similarity of the epidemic S. typhi strains, there was low similarity (F value0.2-0.258) between group A strains and group B strains. However, the subgroup strains (A1-A4) showed high similarity (F value0.8-0.897) each other. The drug susceptibility test showed susceptibility to ampicillin, carbenicillin, cephalothin, chloramphenicol, tobramycin, gentamicin, kanamycin, nalidixic acid, neomycin, polymyxin B, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, ciprofloxacin and norfloxacin.CONCLUSION:
This study showed that phage typing and PFGE were very useful as a tool to investigate molecular analyses of epidemic S. typhi strains this study.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimixina B
/
Salmonella
/
Salmonella typhi
/
Tetraciclina
/
Tobramicina
/
Bacteriófagos
/
Febre Tifoide
/
DNA
/
Carbenicilina
/
Gentamicinas
Idioma:
Coreano
Revista:
Korean Journal of Infectious Diseases
Ano de publicação:
1997
Tipo de documento:
Artigo
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