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Effects of Alpha-lipoic Acid on Nitric Oxide Synthase Expression and Ultrastructural Changes in the Bladder of Rats with Streptozotocin-induced Diabetes / 대한비뇨기과학회지
Korean Journal of Urology ; : 212-218, 2007.
Artigo em Inglês | WPRIM | ID: wpr-116812
ABSTRACT

PURPOSE:

To evaluate whether alpha-lipoic acid (ALA) is effective at restoring the levels of nitric oxide synthase (NOS) expression and preventing ultrastructural changes in the bladder of rats with streptozotocin- induced diabetes. MATERIALS AND

METHODS:

Nine-week-old male Sprague-Dawley rats were used. The experimental groups included a control group (n=6), a diabetes group (n=6), and two groups of diabetic rats treated with intraperitoneal injections of ALA (n=12) at either 50 (ALA50) or 100mg/kg/day (ALA100) for 8 weeks after the induction of diabetes. Diabetic oxidative stress was determined based on evaluation of immunohistochemical staining for 8-hydroxy-2-deoxyguanosine (8-OHdG). The measurements of the levels of eNOS and nNOS expressions, as well as an assessment of the ultrastructural changes in detrusor smooth muscle cells were performed.

RESULTS:

The highest expression of 8-OHdG was observed in the diabetes group; whereas, the 8-OHdG expression in the ALA-treated groups was similar to that in the control group. Both eNOS and nNOS were constitutively expressed in the control group. The expression levels of both eNOS and nNOS proteins were higher in the diabetes group, which had experienced increased oxidative stress, than in the ALA50 and ALA100 groups. Compared with the control group, the diabetes group exhibited severe degeneration of the detrusor muscle cells. In the rats treated with ALA, the detrusor muscle cells showed mild to moderate degeneration. The mean numbers of mitochondria per smooth muscle cell in the control, diabetes, ALA50 and ALA100 groups were 12.6+/-1.5, 5.1+/-0.7, 18.3+/-0.7 and 19.3+/-1.3, respectively (p<0.01). CONCLISIONS Our data suggest that diabetes enhanced the levels of eNOS and nNOS expressions in the bladder, and ALA inhibited the expressions of eNOS and nNOS. ALA had a protective effect against the degeneration of intracellular micro-organelles produced by diabetic oxidative damage in detrusor muscle cells. This study suggests that early treatment with ALA can reduce the damage caused by diabetic oxidative stress.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Bexiga Urinária / Ácido Tióctico / Ratos Sprague-Dawley / Estresse Oxidativo / Óxido Nítrico Sintase / Células Musculares / Miócitos de Músculo Liso / Diabetes Mellitus / Injeções Intraperitoneais / Mitocôndrias Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Korean Journal of Urology Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Bexiga Urinária / Ácido Tióctico / Ratos Sprague-Dawley / Estresse Oxidativo / Óxido Nítrico Sintase / Células Musculares / Miócitos de Músculo Liso / Diabetes Mellitus / Injeções Intraperitoneais / Mitocôndrias Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Korean Journal of Urology Ano de publicação: 2007 Tipo de documento: Artigo