Effect of Apocynin on Acute Lung Injury in Rats Given Interleukin-1alpha Intratracheally / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 482-489, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-117511
ABSTRACT
BACKGROUND:
Based on the assertion that apocynin diminishes acute lung injury (ALI) by inhibition of NADPH oxidase, the effect of apocynin was tested in interleukin-1alpha (IL-1)-induced ALI in rats.METHODS:
IL-1 was insufflated into the trachea of Sprague-Dawley rats to induce ALI, and apocynin (8 mg/kg) was given intravenously for inhibition of NADPH oxidase. In addition, we determined whether apocynin inhibited generation of superoxide anions from isolated human neutrophils. Five hours after IL-1 instillation, lung injury parameters, expression of cytosolic phospholipase A2 (cPLA2) by cells from bronchoalveolar lavage (BAL), an index of oxidative stress in lung tissues (gamma-glutamyltranspeptidase, activity), and ultrastructure of alveolar type II (AT II) cells were evaluated.RESULTS:
Apocynin decreased the generation of free radicals from phorbol myristate (PMA)-activated neutrophils in vitro, but did not ameliorate ALI. IL-1 induced enhancement of the expression of cPLA2 on neutrophils was not altered by apocynin.CONCLUSION:
Apocynin induced suppression of the generation of superoxide anions from neutrophils by inhibition of NADPH oxidase does not attenuate IL-1-induced ALI in rats.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Acetofenonas
/
Traqueia
/
Forbóis
/
Interleucina-1
/
Ratos Sprague-Dawley
/
Superóxidos
/
Estresse Oxidativo
/
Lavagem Broncoalveolar
/
NADPH Oxidases
/
Ácido Mirístico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Tuberculosis and Respiratory Diseases
Ano de publicação:
2011
Tipo de documento:
Artigo
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