Na+i, K+i and Cl-i regulation of exocytosis in guinea-pig antral mucous cells
Journal of Korean Medical Science
;
: S36-S37, 2000.
Artigo
em Inglês
| WPRIM
| ID: wpr-117529
ABSTRACT
Effects of intracellular Na+, K+ and Cl- on Ca(2+)-regulated exocytosis activated by 10 microM acetylcholine (ACh) were studied in guinea-pig antral mucous cells which are permeabilized by nystatin treatment. Ca(2+)-regulated exocytotic events were modulated by [Na+]i, [K+]i and [Cl-]i via mediation of PTX-sensitive G proteins. Increases in [Na+]i and PTX inhibit G protein (G(Na)), which suppressed the exocytosis. Increases in [K+]i caused the exchange of G proteins (from G(Na) to G(K)) to increase, and GK evoked activation of the exocytosis and was inhibited by PTX. Increases in [Cl-]i and PTX inhibit G protein (G(Cl)), which stimulates exocytotic events. Based on these observations, the exocytosis in antral mucous cells were modulated by intracellular ions, concentration of which were increased or decreased by cell volume changes caused by Ach.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Potássio
/
Antro Pilórico
/
Vasodilatadores
/
Cloreto de Sódio
/
Acetilcolina
/
Permeabilidade da Membrana Celular
/
Nistatina
/
Toxina Pertussis
/
Exocitose
/
Mucosa Gástrica
Idioma:
Inglês
Revista:
Journal of Korean Medical Science
Ano de publicação:
2000
Tipo de documento:
Artigo
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