Human Platelet Antigen Genotyping Using a Multiplex Single-Base Primer Extension Reaction in Koreans / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 147-154, 2013.
Artigo
em Coreano
| WPRIM
| ID: wpr-117794
ABSTRACT
BACKGROUND:
Alloimmunization of human platelet antigens (HPA) is associated with clinically significant disease, such as platelet refractoriness, neonatal alloimmune thrombocytopenia, or posttransfusion purpura. It is determined by single nucleotide polymorphism of genes for platelet membrane glycoprotein. To date, approximately 27 HPAs have been discovered, and their frequencies differ depending on ethnicity and country.METHODS:
We conducted an investigation of prevalence of HPA in the Korean population using a multiplex single-base primer extension reaction (SNaPshot). With 84 specimens from healthy donors, HPA genotyping was performed on 11 different HPAs, including HPA-1, -2, -3, -4, -5, -6, -7, -8, -9, -13, and -15.RESULTS:
A total of 90 blood samples were genotyped. The genotype frequencies of HPA were as follows HPA-1a/1a 100.0%, -2a/2a 83.3%, -2a/2b 14.3%, -2b/2b 2.4%, -3a/3a 39.3%, -3a/3b 52.4%, -3b/3b 8.3%, -4a/4a 100.0%, -5a/5a 95.2%, -5a/5b 4.8%, -6a/6a 94.0%, -6a/6b 6.0%, -7a/7a 100.0%, -8a/8a 100.0%, -9a/9a 97.6%, -9a/9b 2.4%, -13a/13a 100.0%, -15a/15a 23.8%, -15a/15b 51.2%, and -15b/15b 25.0%.CONCLUSION:
The SNaPshot assay was employed for detection of SNPs in various clinically significant HPA genes. In addition to well-known frequencies of previously reported HPA-1 to -8, this study showed frequencies of HPA-9, -13, and -15 in Koreans for the first time. The SNaPshot technique might be suitable for use in actual clinical testing in patients with platelet alloimmunization.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Púrpura
/
Púrpura Trombocitopênica
/
Doadores de Tecidos
/
Plaquetas
/
Glicoproteínas de Membrana
/
Prevalência
/
Antígenos de Plaquetas Humanas
/
Polimorfismo de Nucleotídeo Único
/
Trombocitopenia Neonatal Aloimune
/
Genótipo
Tipo de estudo:
Estudo de prevalência
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Blood Transfusion
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS