Expression of TLR-4 in epithelial serous ovarian cancer / 부인종양
Korean Journal of Gynecologic Oncology
;
: 180-186, 2007.
Artigo
em Coreano
| WPRIM
| ID: wpr-119005
ABSTRACT
OBJECTIVE:
This study was to determine the expression of Toll-like receptor 4 (TLR-4) in ovarian serous adenocarcinoma tissues.METHODS:
TLR-4 expression was evaluated at the RNA level by real-time quantitative RT-PCR, in 24 fresh frozen ovarian serous adenocarcinoma tissues and 9 normal ovarian tissues. TLR-4 expression was also evaluated by immunohistochemistry (IHC) in each three ovarian carcinoma tissues and normal ovarian tissues.RESULTS:
Positive immunoreactivity for TLR-4 was observed in the normal ovarian tissues but not in the ovarian carcinoma tissues. The staining was localized in the cytoplasm as well as on the cell surface. Real-time quantitative RT-PCR revealed that TLR-4 expression was significantly lower in tumors than in normal ovarian tissues (p=0.0003). There were no significant correlations between clinical parameters and the expression level of TLR-4 mRNA in ovarian serous adenocarcinomas. However, tumors without LN metastasis (p=0.068) and lower grade (p=0.075) showed trends of higher TLR-4 mRNA expression.CONCLUSION:
TLR-4 expression was significantly lower in ovarian serous adenocarcinoma tissues than in normal ovarian tissues, and further studies on TLR-4 signaling pathway in ovarian carcinoma are needed.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Ovarianas
/
RNA
/
RNA Mensageiro
/
Imuno-Histoquímica
/
Adenocarcinoma
/
Citoplasma
/
Receptor 4 Toll-Like
/
Metástase Neoplásica
Idioma:
Coreano
Revista:
Korean Journal of Gynecologic Oncology
Ano de publicação:
2007
Tipo de documento:
Artigo
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