Ascorbic Acid Enhances Nitric Oxide Production in Trabecular Meshwork Cells
Korean Journal of Ophthalmology
;
: 227-232, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-119100
ABSTRACT
PURPOSE:
This study investigated the role of ascorbic acid on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells.METHODS:
After primarily cultured human TM cells were exposed to 1, 10, and 100 micrometer of L-ascorbic acid (LAA), with or without co-administration of 1 mM sodium nitroprusside or 100 micrometer hydrogen peroxide for 48 hr, cellular survival and NO production were measured with MTT and Griess assay, respectively.RESULTS:
LAA significantly potentiated NO production in a dose-dependent manner (p< 0.05) without affecting cell viability. LAA increased cell viability after hydrogen peroxide-induced oxidative stress in a dose-dependent manner. LAA enhanced NO production in TM cells and showed a cytoprotective effect against hydrogen peroxide-induced oxidative stress.CONCLUSIONS:
LAA might be involved in the regulation of trabecular outflow by enhancing NO production in TM cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ácido Ascórbico
/
Malha Trabecular
/
Sobrevivência Celular
/
Células Cultivadas
/
Relação Dose-Resposta a Droga
/
Óxido Nítrico
Limite:
Humanos
Idioma:
Inglês
Revista:
Korean Journal of Ophthalmology
Ano de publicação:
2005
Tipo de documento:
Artigo
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